Asian Journal of Immunology

Molecular Analysis of GSTP1 ILE105VAL Polymorphism and Its Association with Gastric Cancer Susceptibility

Artemis Socorro do N. Rodrigues *

Laboratory of Molecular Biology and Biotechnology, Federal University of Amapá, Federal University of Amapá, Brazil.

Ana Rita de Cássia B. Miranda

Laboratory of Molecular Biology and Biotechnology, Federal University of Amapá, Federal University of Amapá, Brazil.

Carla Amorim

Laboratory of Molecular Biology and Biotechnology, Federal University of Amapá, Federal University of Amapá, Brazil.

Yasmin Maria N. Cardoso

Graduate Program of Pharmacy, Federal University of Amapá, Brazil.

*Author to whom correspondence should be addressed.

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Abstract

This study was aimed at examining the association between GSTP1 Ile105Val polymorphism and gastric cancer in the city of Macapá, state of Amapá, Amazon, Brazil. Overall 160 DNA samples, 62 cases and 98 controls, were examined using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) with the restriction enzyme BsmAI to detect polymorphism. Our results revealed that 79.0% (X²=86,077, p<0.0001) of the cases with gastric cancer had the ile105val genotype compared to 7.1% of control samples. GSTP1 polymorphism frequency in exon 5 in cases and controls was 83.8% and 15.3%,respectively, for the AG+GG alleles (p <0.0001, X²=73. 347). Our results suggest that GSTP1 Ile105Val polymorphism may contribute to gastric cancer susceptibility in the study population because the mutation in the GSTP1 gene alters the enzymatic function of GSTP1, which might lead to the development of the disease.

Keywords: GSTP1, genetic polymorphisms, gastric cancer

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