Assessment of Post Exposure of Benzene on Some Hematology Parameters and DNA Lesions on Adult Wistar Rats
Orisadiran Patrick Kunle *
Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Adedeji Adebayo Lawrence
Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Adedeji Abiodun Oluwabusola
Department of Anatomy, Olabisi Onabano University, Ago Iwoye, Nigeria.
AbdulRahman AbdulFatai
Department of Anatomy, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: The study sought to investigate the effect of post-exposure of benzene on some haematology parameters and DNA lesions on adult wistar rats.
Methods: A total of twenty-eight rats were grouped into 4 groups, with group 1 serving as a control. The remaining 3 groups were interperitoneally administered 0.2 ml of benzene 48 hourly for 4 weeks and were left of 0, 3 and 6 weeks respectively before sacrifice.
Results: The result obtained showed that White Blood Cell (WBC), Red Blood Cell (RBC) and platelet counts were significantly reduced in benzene post exposed groups (p<0.05). 8-hydroxydeoxyguanosine generation in liver and bone marrow were significantly higher in benzene post exposed groups. The photomicrograph of blood film of benzene exposed group showed promyelocyte and myeloblast.
Conclusion: It is concluded that benzene and its metabolite are highly toxic and are potentially damaging agents to the hematopoietic system. Evidence from our study suggested that elevated levels of 8-OHdG in liver and bone marrow compare to control would be a sign of increased oxidative stress, impaired antioxidant defence or inadequate repair of oxidative damaged DNA.
Keywords: Interperitoneally, hematology, 8-hydroxydeoxyguanosine, promyelocyte, myeloblast, benzene.